MiR-193b inhibits the growth and metastasis of renal cell carcinoma by targeting IGF1R

MiR-193b inhibits the growth and metastasis of renal cell carcinoma by targeting IGF1R

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Objective To explore the effects of miR-193b on cell proliferation, migration, invasion and tumourigenicity of renal cell carcinoma, and the underlying molecular mechanisms.Methods The expression of miR-193b and IGF1R was detected by quantitative real-time polymerase chain reaction (qRT-PCR).MTT assay was used to Slip-On detect cell viability.The migration and invasion abilities were measured by transwell assay.Western blot was used to detect the protein expression of IGF1R.

Murine xenograft model was established using Caki-1cells transfected with miR193b.Results The expression of miR-193b was significantly down-regulated in renal cell carcinoma tissues and cells while the expression of IGF1R was obvious increased in tissues.Overexpression miR-193b or knockdown of IGF1R significantly inhibited the abilities of cells proliferation, migration and invasion in renal cell carcinoma.MiR-193b directly targeted IGF1R and inhibited its expression in vitro and vivo.Up-regulation miR-193b inhibits cells proliferation, migration and invasion of renal cell carcinoma by targeting IGF1R.

In addition, overexpression miR-193b significantly inhibited tumour growth Vinyl Record Accessories in nude mice.Conclusion miR-193b can inhibit the growth and metastasis of renal cell carcinoma by targeting decreasing IGF1R expression, which provides a new target for the prevention and treatment of renal cell carcinoma.